Quantitative fluorescence imaging enables the measurement of dynamic changes in real-time, offering a deeper understanding of cellular behavior. Derive meaningful data with sensitive, real-time live cell measurements made inside the stable condition of the incubator. Perform real-time, live-cell imaging and analysis of cell health, movement, morphology and function directly f… Make the complex simple with powerful and integrated live-cell imaging and analysis tools that support your entire research team. Easy-to-use software to support all scientists across variety of cell models – turn complex assays into reliable and objective publication-ready data
The integration of AI and machine learning in live-cell imaging will enable quicker and deeper data mining. By enabling the study of living cells in their natural environment, live-cell imaging offers a more accurate and comprehensive view of biological processes. To ensure the best possible image quality, researchers must optimize various settings such as exposure time, focus, and illumination intensity. Optimizing assays for extended kinetic experiments is key to maintaining cell health and ensuring high-quality, reproducible imaging.
- These software solutions often include advanced features such as real-time image processing, customizable analysis, and detailed reporting tools.
- As we continue to push the boundaries of what is possible with live-cell imaging, we move closer to a future where we can predict and treat diseases with unprecedented precision and efficacy.
- These systems typically do not sit within a tissue culture incubator and so are limited in terms of environmental stability and long-term studies.
- To ensure the best possible image quality, researchers must optimize various settings such as exposure time, focus, and illumination intensity.
- We provide remote support, additional training and other servic…
- Cells are dynamic in nature, so it is important to have the ability to image in real time to gain access to deeper biological insights.
Do all three Incucyte® systems support the same fluorescent channels?
This capability is essential for capturing transient events and understanding the temporal dynamics of cellular functions. Fluorescence microscopy allows for precise quantitative measurements of various cellular processes. This results in high-contrast images that clearly delineate different structures within the cell, making it easier to study their interactions and functions. Fluorescence microscopy enhances the visibility of cellular components by using fluorescent dyes or proteins that emit light when excited by specific wavelengths. Long-term live-cell imaging often involves repetitive tasks that can be efficiently managed through automation. Advanced environmental chambers and incubators are essential for creating stable and consistent conditions that support long-term cell culture.
Incucyte® S3 Features
The integration of 3D cell culture further enhances this approach by mimicking the structural complexity of living tissues, offering deeper insights into development, disease, and therapeutic response. Live-cell imaging has become essential for studying the dynamic biology of living cells. Make new discoveries with a wide range of applications — from complex immune-tumor cell interactions, synaptic activity in neuronal co-cultures, metabolism in cancer cells, and much more.
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Live-Cell Imaging and Analysis Support and Service
Automated systems in live-cell analysis streamline image acquisition and processing, enabling efficient, accurate, and reproducible results. Fluorescence microscopy systems are designed to acquire images at high speeds, enabling researchers to observe rapid changes and dynamic processes in real-time. By tagging specific molecules with fluorescent markers, researchers can track their movement, concentration, and interactions within the cell, providing valuable data for understanding cellular mechanisms. The ability to selectively label and visualize specific molecules provides valuable insights into cellular processes that would otherwise be invisible. Efficient data management systems and advanced analytical tools are essential to handle this influx of information, enabling researchers to extract meaningful insights without being overwhelmed by the data.
The integrated software simplifies data analysis to accelerate response time, including the creation of publication-quality graphs and charts. Capture high-resolution fluorescence and brightfield images, recording data in real-time over hours, days, or weeks. Cells are dynamic in nature, so it is important to have the ability to image in real time to gain access to deeper biological insights. Traditional end-point assays only provide single measurement of cellular events. Live-cell imaging goatz casino no deposit bonus and analysis should be used when studying any area of cell therapeutics.
- Fluorescence microscopy allows for precise quantitative measurements of various cellular processes.
- The volume of data generated during long-term imaging experiments can be substantial, posing challenges in storage, processing, and analysis.
- Spend more time investigating and less time troubleshooting with lab-tested protocols and purpose-built, intuitive software
- Incucyte® S3 shares the same highest maximum throughput as the Incucyte® SX5 but supports only the phase channel as well as green and red fluorescence.
- Live-cell imaging of T cells engineered to target and kill GFP labelled tumor cells.
- Generate data rich information from every sample with fluorescent reagent combinations – compatible with a variety of cell culture models and applications
These systems typically do not sit within a tissue culture incubator and so are limited in terms of environmental stability and long-term studies. As such, it becomes easier to target internal cellular structures with dyes and antibodies. The fixation process kills the cells, which makes the outside cell membrane more permeable than living cells. While cellular composition is compromised, larger structures such as proteins, organelles and DNA are conserved. Fixed cells have been preserved by a fixation step that “locks” the cell in place.
Make informed decisions about your cultures, rapidly optimize and improve your workflows, plus study complex live-cell assays to fast track your next discovery! Many technologies such as multi-mode readers and high-content imagers do not have the ability to maintain environmental control, meaning cells are not kept at physiologically relevant conditions. With advancements in modern high-resolution imaging techniques and the synthesis of fluorescent probes, it is now possible to view labeled sub-cellular structures at the nm scale. The Incucyte® Live-Cell Analysis System automatically monitors cells for days, weeks or even months as they sit stationary in the stable tissue culture incubator environment. Get answers to the most common questions about about Sartorius image and analysis solutions including critical information regarding instruments, applications, software, service, and more. Making advanced live-cell imaging technology accessible to all researchers is of critical importance.
The content of our website is always available in English and partly in other languages. See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data. Incucyte® S3 shares the same highest maximum throughput as the Incucyte® SX5 but supports only the phase channel as well as green and red fluorescence. For the Incucyte® S3, an incubator larger than 200L is recommended.
To ensure accurate representation of cellular dynamics, it's important to optimize acquisition speed, resolution, and time intervals based on the specific biological phenomena being studied. However, over-collection of images can lead to excess data, increasing processing time and storage demands without necessarily enhancing biological insight. The ability to quickly capture images is crucial for studying fast kinetics in cellular activities.